To investigate the breeding system of plant species, Amos Dafni provided different pollination treatments. Using these one can disentangle if a species is self-compatible or depending on outcross-pollination. Furthermore, one can get initial sights of inbreeding depression and if a plant species is pollen or resource limited. Emasculation means cutting the anthers (carrying the pollen grains). Bagging is to be done by mesh nets or perforated pollen proof pollination bags. Hand-pollination can be carried out by using paint brushes or tooth picks.

The number of damaged (= non intact, not fully developed or aborted seeds, that are not viable) was significantly increased after spontaneous self-pollination than after cross pollination. This is a clear sign of inbreeding depression in the lack of pollinators and economically relevant, since the seeds are used for the next year sowing season. In a nutshell: self-pollination decreases seed quality of cotton.